GastroPlus 7.0 is designed to run on Pentium-class Windows XP and Windows Vista computers. Windows 7 is not officially supported at this time, but internal test installations did not uncover any issues. A minimum of 256 MB of RAM and screen resolution of 600x800 pixels is recommended for optimal performance.

Most modern Pentium machines with speeds of 133 MHz or faster should be able to run GastroPlus; however, simulation software is computationally intensive and always benefits from greater speed and memory. On a 2.4 GHz Pentium 4 computer with 512 MB of RAM, GastroPlus executes a typical 24-hour single simulation in less than one second with standard compartmental pharmacokinetics. Because they incorporate a much larger set of differential equations, PBPK simulations typically require about 10-20 seconds, but can be longer. Simulations that include particle size distributions with a large number of bins can also require more time because they also require a larger set of differential equations to be numerically integrated.