Using Many In Vitro Assay Systems to Make a Kidney Toxicity Prediction of Doxorubicin: A Proof of Concept in Support of the New Approach Methodologies Initiative

Authors: C Vallejo, Woodhead JL
Conference: ACoP
Keywords: ACoP 2026, kidney toxicity, New Approach Methodologies (NAMs), Quantitative Systems Toxicology (QST), RENAsym
Software: RENAsym®

Objective

  • With the introduction of the new approach methodologies (NAMs) initiative, there is a focus on finding alternatives to animal studies to validate a drug’s safety before administering in humans.
  • Quantitative systems toxicology (QST) models are uniquely positioned to address this need. However, these models often rely on inputs from in vitro data with the ideal not always available.
  • In this work, kidney toxicity predictions were made using information gathered in a variety of cell lines (kidney and others).
  • The objective of this proof of concept was to find ways to strengthen the predictive ability of QST models using less-than-ideal in vitro inputs.

By Celeste Vallejo and Jeffrey L. Woodhead

ACoP 2026, October 11-14, 2026, National Harbor, Maryland