in vitro permeability & hepatocyte modeling software

Choose a Module:

Your simulation tool for IVIVE of absorption processes…

For over 20 years, significant research has been devoted to in vitro permeability studies (e.g., Caco-2 assays), with several guidelines being published and all pharmacopeia describing appropriate methods for testing. Unfortunately, the permeability experiments are relatively expensive, and since you don’t have a lot of time or material available, how do you prioritize which compounds to test early in your project? Or, once you have identified a few promising leads, is your experimental design robust enough to capture all of the relevant information you need?

Enter MembranePlus™, the industry’s leading mechanistic in vitro permeability software. MembranePlus is helpful for:

  • Users of GastroPlus™ looking to define inputs (e.g., lysosomal trapping and unbound fractions) for more accurate in vivo absorption predictions in the Advanced Compartmental Absorption and Transit (ACAT™) model
  • Discovery scientists looking to categorize compounds into high and low permeability classes
  • DMPK researchers that focus on in vitro transporter/metabolism kinetic studies
  • CROs which provide in vitro Caco-2, PAMPA, or MDCK permeability services, to assist with the optimization of the experimental design for client studies

The two modules of MembranePlus™ are:

What are we providing with MembranePlus?

We have always been dedicated to carefully implement the best theories and develop novel approaches within the MembranePlus permeability models. You enter limited physicochemical & metabolism/transporter data (if applicable), set up your permeability experiment, and MembranePlus handles the rest:

  • Simple, intuitive user interface
  • Model optimization
  • High-quality plots & figures for reporting purposes
  • Excellent customer support
  • Integration with our other tools, like ADMET Predictor™ and GastroPlus

The in vitro processes which are considered in the MembranePlus simulations, and the methods we’ve introduced to parameterize them, are too numerous to list here – instead, take a peek at the MembranePlus datasheet for more details.

How can MembranePlus help me?

The MembranePlus permeability modeling platform has been utilized by companies across various industries and departments since 2014. MembranePlus has two primary applications:

Simulation of in vitro permeability

  • Screen compound libraries and prioritize compounds for testing
  • Predict various permeability processes (e.g., paracellular, transcellular)
  • Estimate different intracellular concentrations, including:
    • Membrane
    • Cytosol
    • Lysosome
  • Assess impact of experimental variability on the predicted outcomes

Analysis of measured in vitro permeability data

  • Identify paracellular vs. transcellular permeability values
  • Calculate in vitro Km and Vmax for enzymes and transporters
  • Determine the impact of lysosomal trapping
  • Fit parameters to build a robust model and unlock important insight from the data

Check out various case studies using MembranePlus and GastroPlus by reviewing publications and webinars on our Resource Center.

How do I move forward from here?

Join your peers around the world and begin working with MembranePlus to unlock important information from your in vitro permeability (Caco-2, PAMPA, and MDCK cells) and hepatocyte studies.

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